1A8-CP129单克隆抗体是原始1A8单克隆抗体的重组嵌合型抗体。可变结构域序列与原始1A8相同，但是恒定区序列已经从大鼠IgG2a变为小鼠IgG2a。1A8-CP129单克隆抗体像原始克隆号的大鼠IgG2a抗体一样，不包含Fc突变。
1A8-CP129单克隆抗体与小鼠Ly6G反应。Ly6G分子量为21-25kDa，是GPI锚定的细胞表面蛋白Ly-6超家族的成员，在细胞信号传导和细胞粘附中发挥作用。Ly6G在发育过程中由骨髓谱系中的细胞（包括单核细胞、巨噬细胞、粒细胞和嗜中性粒细胞）差异表达。单核细胞通常在发育过程中瞬时表达Ly6G，而成熟的粒细胞和外周嗜中性粒细胞持续表达，使Ly6G成为这些细胞群体的表面标志物。与BioXcell RB6-8C5单克隆抗体不同，1A8-CP129单克隆抗体与小鼠Ly6G特异性反应，而与Ly6C没有交叉反应性的报道。
产品详情：
| 产品名称 | RecombiMAb anti-mouse Ly6G/欣博盛生物 |
| 产品货号 | CP129 |
| 产品规格 | 1/5/25/50/100mg |
| 反应种属 | Mouse |
| 克隆号 | 1A8-CP129 |
| 同种型 | Mouse IgG2a(switched from rat IgG2a) |
| 免疫原 | EL4J cells transfected with Ly6G |
| 实验应用 | in vivo neutrophil depletion* in vivo MDSC depletion* Immunofluorescence* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* Flow cytometry* *Reported for the original rat IgG2a 1A8 antibody |
| 产品形式 | PBS, pH 7.0,Contains no stabilizers or preservatives |
| 纯度 | >95%, Determined by SDS-PAGE |
| 聚合 | <5%, Determined by SEC |
| 无菌处理 | 0.2 µm filtration |
| 纯化方式 | Protein G |
| 分子量 | 150 kDa |
| 小鼠病原检测 | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| 保存条件 | 抗体原液保存在4°C，不能冷冻保存。 |
| 推荐同型对照 | InVivoPlus mouse IgG2a isotype control, unknown specificity（货号BP0085） |
| 推荐抗体稀释液 | InVivoPure pH 7.0 Dilution Buffer（货号IP0070） |
欣博盛生物是Bioxcell授权国内一级代理，如需购买该产品或咨询其它产品/技术问题，请联系Bioxcell代理商欣博盛生物。
该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：
| 应用 | 文章 |
| 体内中性粒细胞耗竭 (in vivo neutrophil depletion) | 1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic Efficacy' Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses' Nat Med. doi : 10.1038/nm.4200. 3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation Tolerance' Immunity 42(6): 1143-1158. 4. Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection' Immunity 42(2): 379-390. |
| 体内中性粒细胞耗竭、流式细胞术、 免疫组化石蜡切片(in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin)) | Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis' Nature 522(7556): 345-348. |
| 体内中性粒细胞耗竭、流式细胞术、 免疫组化石蜡切片、免疫组化冰冻切片 (in vivo neutrophil depletion, Flow Cytometry, Immunohistochemistry (paraffin), Immunohistochemistry (frozen) | Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural neutrophils' Nature 522(7556): 349-353. |
| 体内中性粒细胞耗竭、流式细胞术 (in vivo neutrophil depletion, Flow Cytometry) | 1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner' PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively promotes IL-1beta maturation without pyroptosis during acute Salmonella challenge' Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142. |
| 体内骨髓来源抑制细胞耗竭 (in vivo MDSC depletion) | Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically promote antitumor immunity in mice' J Clin Invest 124(2): 687-695. |
| 体内中性粒细胞耗竭、 免疫荧光(in vivo neutrophil depletion, Immunofluorescence) | Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes' Immunity 35(2): 236-248. |
