Histone H3K27ac Antibody, SNAP-ChIP® Certified-编程知识

Histone H3K27ac Antibody, SNAP-ChIP® Certified

EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher（国内代理商欣博盛生物）推出的ChIP级别的Histone H3K27ac Antibody符合EpiCypher的“SNAP-ChIP® Certified”标准，用于ChIP实验中的特异性和有效靶标富集（整板的交叉反应性<20%，靶标输入的回收率>5%）。组蛋白H3是核小体中存在的四种蛋白质之一，核小体是染色质的基本重复亚基，有147个碱基对的DNA缠绕在核心组蛋白（H2A、H2B、H3和H4）的八聚体上。该抗体与H3K27ac结合，并且在EpiCypher SNAP-ChIP K-AcylStat Panel（EpiCypher 19-3001）中未检测到与其他赖氨酸酰化的显著交叉反应性。在S28（H3K27acS28ph）磷酸化的情况下，抗体与H3K27ac的结合在ChIP中受到不同程度的抑制（图3）。

产品详情

反应种属: Human, Mouse, Wide Range (Predicted)

宿主来源: Mouse

实验应用: ChIP, ICC/IF

免疫原: A synthetic peptide corresponding to histone H3 acetylated at lysine 27

克隆性: Monoclonal

保存温度: Stable for 1 year at -20°C from date of receipt

运输温度: Frozen cold packs.

产品形式: Protein A affinity-purified antibody in PBS pH 7.4, 0.05% sodium azide

验证数据

Epicypher热销产品——Histone H3K27ac Antibody, SNAP-ChIP® Certified

Figure 1:Representative SNAP-ChIP-seq results: Cumulative histogram plot and heatmap of signal intensity depict H3K27ac ChIP-seq data aligned to annotated transcription start sites (TSS, +/- 3.0 kb; left). Two representative genomic regions depicting H3K27ac peak structure and distribution are shown (right).

Epicypher热销产品——Histone H3K27ac Antibody, SNAP-ChIP® Certified

Figure 2: SNAP-ChIP-qPCR data. Histone H3K27ac antibody (3 µg) was tested in a native ChIP experiment using chromatin from K562 cells (3 µg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left y-axis) was determined by qPCR for the DNA barcodes corresponding to modified nucleosomes in the SNAP-ChIP panel (x-axis). Black bar represents antibody efficiency (right yaxis; log scale) and indicates percentage of the target immunoprecipitated relative to input. Error bars represent mean ± SEM in replicate ChIP experiments.

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