AF700 NHS 酯，AF 700 Succinimidyl Ester，一种明亮且具有光稳定性的近红外染料-编程知识

AF700 NHS 酯，AF 700 Succinimidyl Ester，一种明亮且具有光稳定性的近红外染料

AF700 NHS 酯，AF 700 Succinimidyl Ester，一种明亮且具有光稳定性的近红外染料，AF700-NHS-酯，具有水溶性和 pH 值不敏感性

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文章关键词：AF700 NHS 酯，AF 700 Succinimidyl Ester，AF700-NHS-酯

一、基本信息

产品简介：AF 700 is a bright and photostable near-infrared dye that can be excited using xenon arc lamps, far-red diode lasers, or dye pumped lasers with a working spectral range of 675-700 nm. AF 700 dye is used for stable signal generation in imaging and flow cytometry analysis, with water solubility and pH insensitivity (pH 4 to pH 10). The fluorescence of this long wavelength AF dye is invisible to the human eye, but it is easily detected by most imaging systems. In addition to reactive dye formulations, we also offer AF 700 dyes that can be coupled with various antibodies, peptides, proteins, tracers, and amplification substrates and optimized for cell labeling and detection.

AF700 NHS ester (succinimide ester) is the same as AlexaFluor700 NHS ester. It is a bright red fluorescent dye. AF700 dye is water-soluble and insensitive to pH 4 to pH 10 The NHS ester (or succinimide ester) of AF700 is a convenient amine reaction form used to bind the dye to proteins or antibodies.

AF 700染料是一种明亮且具有光稳定性的近红外染料，它的激发光谱范围广泛，可以使用氙弧灯、远红二极管激光器或染料泵浦激光器进行激发。这种染料在水溶液中表现出良好的溶解性，并且对pH值具有高度的耐受性，可以在pH值4至pH值10的范围内保持稳定。由于其独特的荧光特性，AF 700染料的荧光光谱位于人眼不可见的波长范围内，但可以被大多数成像系统轻易检测到。
AF 700染料还可以与多种生物分子进行偶联，如抗体、肽、蛋白、示踪剂和扩增底物等。这种偶联使得AF 700染料成为一种理想的荧光标记物，用于细胞标记和检测等多种生物应用。其中，AF700 NHS酯（琥珀酰亚胺酯）是一种特别方便的胺反应形式，可以与蛋白质或抗体上的氨基基团进行快速而有效的偶联。

中文名：AF700 NHS 酯，AF700-NHS-酯

英文名：AF 700 Succinimidyl Ester

Ex (nm)：696

Em (nm)：719

分子量：1400

包装：